| 15. | M Isselstein, L Zhang, V Glembockyte, O Brix, G Cosa, P Tinnefeld, T Cordes Self-Healing Dyes—Keeping the Promise? Journal Article The Journal of Physical Chemistry Letters, 11 (11), pp. 4462-4480, 2020. Links | Tags: Foundry Organic @article{, title = {Self-Healing Dyes\textemdashKeeping the Promise?}, author = {M Isselstein and L Zhang and V Glembockyte and O Brix and G Cosa and P Tinnefeld and T Cordes}, url = {https://doi.org/10.1021/acs.jpclett.9b03833}, doi = {10.1021/acs.jpclett.9b03833}, year = {2020}, date = {2020-06-04}, journal = {The Journal of Physical Chemistry Letters}, volume = {11}, number = {11}, pages = {4462-4480}, keywords = {Foundry Organic}, pubstate = {published}, tppubtype = {article} } |
| 14. | S S Matikonda, G Hammersley, N Kumari, L Grabenhorst, V Glembockyte, P Tinnefeld, J Ivanic, M Levitus, M J Schnermann Impact of Cyanine Conformational Restraint in the Near-Infrared Range Journal Article The Journal of Organic Chemistry, 2020, ISSN: 0022-3263. Links | Tags: Foundry Organic, Molecularly Functionalized @article{, title = {Impact of Cyanine Conformational Restraint in the Near-Infrared Range}, author = {S S Matikonda and G Hammersley and N Kumari and L Grabenhorst and V Glembockyte and P Tinnefeld and J Ivanic and M Levitus and M J Schnermann}, url = {https://doi.org/10.1021/acs.joc.0c00236}, doi = {10.1021/acs.joc.0c00236}, issn = {0022-3263}, year = {2020}, date = {2020-04-10}, journal = {The Journal of Organic Chemistry}, keywords = {Foundry Organic, Molecularly Functionalized}, pubstate = {published}, tppubtype = {article} } |
| 13. | P Mao, C Liu, F Song, M Han, S A Maier, S Zhang Manipulating disordered plasmonic systems by external cavity with transition from broadband absorption to reconfigurable reflection Journal Article Nature Communications, 11 (1), pp. 1538, 2020, ISSN: 2041-1723. Abstract | Links | Tags: Foundry Organic @article{, title = {Manipulating disordered plasmonic systems by external cavity with transition from broadband absorption to reconfigurable reflection}, author = {P Mao and C Liu and F Song and M Han and S A Maier and S Zhang}, url = {https://doi.org/10.1038/s41467-020-15349-y}, doi = {10.1038/s41467-020-15349-y}, issn = {2041-1723}, year = {2020}, date = {2020-03-24}, journal = {Nature Communications}, volume = {11}, number = {1}, pages = {1538}, abstract = {Disordered biostructures are ubiquitous in nature, usually generating white or black colours due to their broadband optical response and robustness to perturbations. Through judicious design, disordered nanostructures have been realised in artificial systems, with unique properties for light localisation, photon transportation and energy harvesting. On the other hand, the tunability of disordered systems with a broadband response has been scarcely explored. Here, we achieve the controlled manipulation of disordered plasmonic systems, realising the transition from broadband absorption to tunable reflection through deterministic control of the coupling to an external cavity. Starting from a generalised model, we realise disordered systems composed of plasmonic nanoclusters that either operate as a broadband absorber or with a reconfigurable reflection band throughout the visible. Not limited to its significance for the further understanding of the physics of disorder, our disordered plasmonic system provides a novel platform for various practical application such as structural colour patterning.}, keywords = {Foundry Organic}, pubstate = {published}, tppubtype = {article} } Disordered biostructures are ubiquitous in nature, usually generating white or black colours due to their broadband optical response and robustness to perturbations. Through judicious design, disordered nanostructures have been realised in artificial systems, with unique properties for light localisation, photon transportation and energy harvesting. On the other hand, the tunability of disordered systems with a broadband response has been scarcely explored. Here, we achieve the controlled manipulation of disordered plasmonic systems, realising the transition from broadband absorption to tunable reflection through deterministic control of the coupling to an external cavity. Starting from a generalised model, we realise disordered systems composed of plasmonic nanoclusters that either operate as a broadband absorber or with a reconfigurable reflection band throughout the visible. Not limited to its significance for the further understanding of the physics of disorder, our disordered plasmonic system provides a novel platform for various practical application such as structural colour patterning. |
| 12. | L Grabenhorst, K Trofymchuk, F Steiner, V Glembockyte, P Tinnefeld Fluorophore photostability and saturation in the hotspot of DNA origami nanoantennas Journal Article Methods and Applications in Fluorescence, 8 (2), pp. 024003, 2020, ISSN: 2050-6120. Abstract | Links | Tags: Foundry Organic, Molecularly-Functionalized @article{, title = {Fluorophore photostability and saturation in the hotspot of DNA origami nanoantennas}, author = {L Grabenhorst and K Trofymchuk and F Steiner and V Glembockyte and P Tinnefeld}, url = {http://dx.doi.org/10.1088/2050-6120/ab6ac8}, doi = {10.1088/2050-6120/ab6ac8}, issn = {2050-6120}, year = {2020}, date = {2020-02-05}, journal = {Methods and Applications in Fluorescence}, volume = {8}, number = {2}, pages = {024003}, abstract = {Fluorescent dyes used for single-molecule spectroscopy can undergo millions of excitation-emission cycles before photobleaching. Due to the upconcentration of light in a plasmonic hotspot, the conditions for fluorescent dyes are even more demanding in DNA origami nanoantennas. Here, we briefly review the current state of fluorophore stabilization for single-molecule imaging and reveal additional factors relevant in the context of plasmonic fluorescence enhancement. We show that despite the improved photostability of single-molecule fluorophores by DNA origami nanoantennas, their performance in the intense electric fields in plasmonic hotspots is still limited by the underlying photophysical processes, such as formation of dim states and photoisomerization. These photophysical processes limit the photon count rates, increase heterogeneity and aggravate quantification of fluorescence enhancement factors. These factors also reduce the time resolution that can be achieved in biophysical single-molecule experiments. Finally, we show how the photophysics of a DNA hairpin assay with a fluorophore-quencher pair can be influenced by plasmonic DNA origami nanoantennas leading to implications for their use in fluorescence-based diagnostic assays. Especially, we show that such assays can produce false positive results by premature photobleaching of the dark quencher.}, keywords = {Foundry Organic, Molecularly-Functionalized}, pubstate = {published}, tppubtype = {article} } Fluorescent dyes used for single-molecule spectroscopy can undergo millions of excitation-emission cycles before photobleaching. Due to the upconcentration of light in a plasmonic hotspot, the conditions for fluorescent dyes are even more demanding in DNA origami nanoantennas. Here, we briefly review the current state of fluorophore stabilization for single-molecule imaging and reveal additional factors relevant in the context of plasmonic fluorescence enhancement. We show that despite the improved photostability of single-molecule fluorophores by DNA origami nanoantennas, their performance in the intense electric fields in plasmonic hotspots is still limited by the underlying photophysical processes, such as formation of dim states and photoisomerization. These photophysical processes limit the photon count rates, increase heterogeneity and aggravate quantification of fluorescence enhancement factors. These factors also reduce the time resolution that can be achieved in biophysical single-molecule experiments. Finally, we show how the photophysics of a DNA hairpin assay with a fluorophore-quencher pair can be influenced by plasmonic DNA origami nanoantennas leading to implications for their use in fluorescence-based diagnostic assays. Especially, we show that such assays can produce false positive results by premature photobleaching of the dark quencher. |
| 11. | M Asher, D Angerer, R Korobko, Y Diskin-Posner, D A Egger, O Yaffe Anharmonic Lattice Vibrations in Small-Molecule Organic Semiconductors Journal Article Advanced Materials, n/a (n/a), pp. 1908028, 2020, ISSN: 0935-9648. Abstract | Links | Tags: Foundry Organic @article{, title = {Anharmonic Lattice Vibrations in Small-Molecule Organic Semiconductors}, author = {M Asher and D Angerer and R Korobko and Y Diskin-Posner and D A Egger and O Yaffe}, url = {https://doi.org/10.1002/adma.201908028}, doi = {10.1002/adma.201908028}, issn = {0935-9648}, year = {2020}, date = {2020-01-31}, journal = {Advanced Materials}, volume = {n/a}, number = {n/a}, pages = {1908028}, abstract = {Abstract The intermolecular lattice vibrations in small-molecule organic semiconductors have a strong impact on their functional properties. Existing models treat the lattice vibrations within the harmonic approximation. In this work, polarization-orientation (PO) Raman measurements are used to monitor the temperature-evolution of the symmetry of lattice vibrations in anthracene and pentacene single crystals. Combined with first-principles calculations, it is shown that at 10 K, the lattice dynamics of the crystals are indeed harmonic. However, as the temperature is increased, specific lattice modes gradually lose their PO dependence and become more liquid-like. This finding is indicative of a dynamic symmetry breaking of the crystal structure and shows clear evidence of the strongly anharmonic nature of these vibrations. Pentacene also shows an apparent phase transition between 80 and 150 K, indicated by a change in the vibrational symmetry of one of the lattice modes. These findings lay the groundwork for accurate predictions of the electronic properties of high-mobility organic semiconductors at room temperature.}, keywords = {Foundry Organic}, pubstate = {published}, tppubtype = {article} } Abstract The intermolecular lattice vibrations in small-molecule organic semiconductors have a strong impact on their functional properties. Existing models treat the lattice vibrations within the harmonic approximation. In this work, polarization-orientation (PO) Raman measurements are used to monitor the temperature-evolution of the symmetry of lattice vibrations in anthracene and pentacene single crystals. Combined with first-principles calculations, it is shown that at 10 K, the lattice dynamics of the crystals are indeed harmonic. However, as the temperature is increased, specific lattice modes gradually lose their PO dependence and become more liquid-like. This finding is indicative of a dynamic symmetry breaking of the crystal structure and shows clear evidence of the strongly anharmonic nature of these vibrations. Pentacene also shows an apparent phase transition between 80 and 150 K, indicated by a change in the vibrational symmetry of one of the lattice modes. These findings lay the groundwork for accurate predictions of the electronic properties of high-mobility organic semiconductors at room temperature. |
| 10. | J Schedlbauer, P Wilhelm, L Grabenhorst, M E Federl, B Lalkens, F Hinderer, U Scherf, S Höger, P Tinnefeld, S Bange, J Vogelsang, J M Lupton Ultrafast Single-Molecule Fluorescence Measured by Femtosecond Double-Pulse Excitation Photon Antibunching Journal Article Nano Letters, 2019, ISSN: 1530-6984. Links | Tags: Foundry Organic, Molecularly-Functionalized @article{, title = {Ultrafast Single-Molecule Fluorescence Measured by Femtosecond Double-Pulse Excitation Photon Antibunching}, author = {J Schedlbauer and P Wilhelm and L Grabenhorst and M E Federl and B Lalkens and F Hinderer and U Scherf and S H\"{o}ger and P Tinnefeld and S Bange and J Vogelsang and J M Lupton}, url = {https://doi.org/10.1021/acs.nanolett.9b04354}, doi = {10.1021/acs.nanolett.9b04354}, issn = {1530-6984}, year = {2019}, date = {2019-12-23}, journal = {Nano Letters}, keywords = {Foundry Organic, Molecularly-Functionalized}, pubstate = {published}, tppubtype = {article} } |
| 9. | K Hübner, M Pilo-Pais, F Selbach, T Liedl, P Tinnefeld, F D Stefani, G P Acuna Directing Single-Molecule Emission with DNA Origami-Assembled Optical Antennas Journal Article Nano Letters, 19 (9), pp. 6629-6634, 2019, ISSN: 1530-6984. Links | Tags: Foundry Organic, Molecularly-Functionalized @article{, title = {Directing Single-Molecule Emission with DNA Origami-Assembled Optical Antennas}, author = {K H\"{u}bner and M Pilo-Pais and F Selbach and T Liedl and P Tinnefeld and F D Stefani and G P Acuna}, url = {https://doi.org/10.1021/acs.nanolett.9b02886}, doi = {10.1021/acs.nanolett.9b02886}, issn = {1530-6984}, year = {2019}, date = {2019-09-11}, journal = {Nano Letters}, volume = {19}, number = {9}, pages = {6629-6634}, keywords = {Foundry Organic, Molecularly-Functionalized}, pubstate = {published}, tppubtype = {article} } |
| 8. | B S Wei, D C Zhang, Y H Chen, A W Lei, P Knochel Preparation of Polyfunctional Biaryl Derivatives by Cyclolanthanation of 2-Bromobiaryls and Heterocyclic Analogues Using nBu(2)LaCl.4 LiCl Journal Article Angewandte Chemie-International Edition, 58 (44), pp. 15631-15635, 2019, ISSN: 1433-7851. Links | Tags: Foundry Organic @article{, title = {Preparation of Polyfunctional Biaryl Derivatives by Cyclolanthanation of 2-Bromobiaryls and Heterocyclic Analogues Using nBu(2)LaCl.4 LiCl}, author = {B S Wei and D C Zhang and Y H Chen and A W Lei and P Knochel}, url = {<Go to ISI>://WOS:000487102700001}, doi = {10.1002/anie.201908046}, issn = {1433-7851}, year = {2019}, date = {2019-08-28}, journal = {Angewandte Chemie-International Edition}, volume = {58}, number = {44}, pages = {15631-15635}, keywords = {Foundry Organic}, pubstate = {published}, tppubtype = {article} } |
| 7. | H Schlomberg, J Kröger, G Savasci, M Terban, S Bette, I Moudrakovski, V Duppel, F Podjaski, R Siegel, J Senker, R Dinnebier, C Ochsenfeld, B V Lotsch Structural Insights into Poly(Heptazine Imides): A Light Storing Carbon Nitride Material for Dark Photocatalysis Journal Article Chemistry of Materials, 31 , 2019. Links | Tags: Foundry Organic @article{, title = {Structural Insights into Poly(Heptazine Imides): A Light Storing Carbon Nitride Material for Dark Photocatalysis}, author = {H Schlomberg and J Kr\"{o}ger and G Savasci and M Terban and S Bette and I Moudrakovski and V Duppel and F Podjaski and R Siegel and J Senker and R Dinnebier and C Ochsenfeld and B V Lotsch}, doi = {10.1021/acs.chemmater.9b02199}, year = {2019}, date = {2019-08-12}, journal = {Chemistry of Materials}, volume = {31}, keywords = {Foundry Organic}, pubstate = {published}, tppubtype = {article} } |
| 6. | C Muschielok, H Oberhofer Aspects of semiconductivity in soft, porous metal-organic framework crystals Journal Article Journal of Chemical Physics, 151 (1), 2019, ISSN: 0021-9606. Links | Tags: Foundry Organic, Molecularly-Functionalized @article{, title = {Aspects of semiconductivity in soft, porous metal-organic framework crystals}, author = {C Muschielok and H Oberhofer}, url = {<Go to ISI>://WOS:000474214600025}, doi = {10.1063/1.5108995}, issn = {0021-9606}, year = {2019}, date = {2019-07-03}, journal = {Journal of Chemical Physics}, volume = {151}, number = {1}, keywords = {Foundry Organic, Molecularly-Functionalized}, pubstate = {published}, tppubtype = {article} } |
| 5. | B P Biswal, H A Vignolo-Gonzalez, T Banerjee, L Grunenberg, G Savasci, K Gottschling, J Nuss, C Ochsenfeld, B V Lotsch Sustained Solar H-2 Evolution from a Thiazolo 5,4-d thiazole-Bridged Covalent Organic Framework and Nickel-Thiolate Cluster in Water Journal Article Journal of the American Chemical Society, 141 (28), pp. 11082-11092, 2019, ISSN: 0002-7863. Links | Tags: Foundry Organic @article{, title = {Sustained Solar H-2 Evolution from a Thiazolo 5,4-d thiazole-Bridged Covalent Organic Framework and Nickel-Thiolate Cluster in Water}, author = {B P Biswal and H A Vignolo-Gonzalez and T Banerjee and L Grunenberg and G Savasci and K Gottschling and J Nuss and C Ochsenfeld and B V Lotsch}, url = {<Go to ISI>://WOS:000476684700023}, doi = {10.1021/jacs.9b03243}, issn = {0002-7863}, year = {2019}, date = {2019-06-20}, journal = {Journal of the American Chemical Society}, volume = {141}, number = {28}, pages = {11082-11092}, keywords = {Foundry Organic}, pubstate = {published}, tppubtype = {article} } |
| 4. | T Banerjee, F Haase, S Trenker, B P Biswal, G Savasci, V Duppel, I Moudrakovski, C Ochsenfeld, B V Lotsch Sub-stoichiometric 2D covalent organic frameworks from tri- and tetratopic linkers Journal Article Nature Communications, 10 , 2019, ISSN: 2041-1723. Links | Tags: Foundry Organic @article{, title = {Sub-stoichiometric 2D covalent organic frameworks from tri- and tetratopic linkers}, author = {T Banerjee and F Haase and S Trenker and B P Biswal and G Savasci and V Duppel and I Moudrakovski and C Ochsenfeld and B V Lotsch}, url = {<Go to ISI>://WOS:000472032300004}, doi = {10.1038/s41467-019-10574-6}, issn = {2041-1723}, year = {2019}, date = {2019-06-19}, journal = {Nature Communications}, volume = {10}, keywords = {Foundry Organic}, pubstate = {published}, tppubtype = {article} } |
| 3. | P Alexa, C Oligschleger, P Gröger, C Morchutt, V Vyas, B V Lotsch, J C Schön, R Gutzler, K Kern Short-Range Structural Correlations in Amorphous 2D Polymers Journal Article ChemPhysChem, 20 (18), pp. 2340-2347, 2019, ISSN: 1439-4235. Abstract | Links | Tags: Foundry Organic @article{, title = {Short-Range Structural Correlations in Amorphous 2D Polymers}, author = {P Alexa and C Oligschleger and P Gr\"{o}ger and C Morchutt and V Vyas and B V Lotsch and J C Sch\"{o}n and R Gutzler and K Kern}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/cphc.201900326}, doi = {10.1002/cphc.201900326}, issn = {1439-4235}, year = {2019}, date = {2019-05-21}, journal = {ChemPhysChem}, volume = {20}, number = {18}, pages = {2340-2347}, abstract = {Abstract Many 2D covalent polymers synthesized as single layers on surfaces show inherent disorder, expressed for example in their ring-size distribution. Systems which are expected to form the thermodynamically favored hexagonal lattice usually deviate from crystallinity and include high numbers of pentagons, heptagons, and rings of other sizes. The amorphous structure of two different covalent polymers in real space using scanning tunneling microscopy is investigated. Molecular dynamics simulations are employed to extract additional information. We show that short-range correlations exist in the structure of one polymer, i. e. that polygons are not tessellating the surface randomly but that ring neighborhoods have preferential compositions. The correlation is dictated by the energy of formation of the ring neighborhoods.}, keywords = {Foundry Organic}, pubstate = {published}, tppubtype = {article} } Abstract Many 2D covalent polymers synthesized as single layers on surfaces show inherent disorder, expressed for example in their ring-size distribution. Systems which are expected to form the thermodynamically favored hexagonal lattice usually deviate from crystallinity and include high numbers of pentagons, heptagons, and rings of other sizes. The amorphous structure of two different covalent polymers in real space using scanning tunneling microscopy is investigated. Molecular dynamics simulations are employed to extract additional information. We show that short-range correlations exist in the structure of one polymer, i. e. that polygons are not tessellating the surface randomly but that ring neighborhoods have preferential compositions. The correlation is dictated by the energy of formation of the ring neighborhoods. |
| 2. | B Garlyyev, K Kratzl, M Rück, J Michalička, J Fichtner, J M Macak, T Kratky, S Günther, M Cokoja, A S Bandarenka, A Gagliardi, R A Fischer Optimizing the Size of Platinum Nanoparticles for Enhanced Mass Activity in the Electrochemical Oxygen Reduction Reaction Journal Article Angewandte Chemie International Edition, 58 (28), pp. 9596-9600, 2019, ISSN: 1433-7851. Abstract | Links | Tags: Foundry Organic, Solid-Liquid @article{, title = {Optimizing the Size of Platinum Nanoparticles for Enhanced Mass Activity in the Electrochemical Oxygen Reduction Reaction}, author = {B Garlyyev and K Kratzl and M R\"{u}ck and J Michali\v{c}ka and J Fichtner and J M Macak and T Kratky and S G\"{u}nther and M Cokoja and A S Bandarenka and A Gagliardi and R A Fischer}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201904492}, doi = {10.1002/anie.201904492}, issn = {1433-7851}, year = {2019}, date = {2019-05-03}, journal = {Angewandte Chemie International Edition}, volume = {58}, number = {28}, pages = {9596-9600}, abstract = {Abstract High oxygen reduction (ORR) activity has been for many years considered as the key to many energy applications. Herein, by combining theory and experiment we prepare Pt nanoparticles with optimal size for the efficient ORR in proton-exchange-membrane fuel cells. Optimal nanoparticle sizes are predicted near 1, 2, and 3 nm by computational screening. To corroborate our computational results, we have addressed the challenge of approximately 1 nm sized Pt nanoparticle synthesis with a metal\textendashorganic framework (MOF) template approach. The electrocatalyst was characterized by HR-TEM, XPS, and its ORR activity was measured using a rotating disk electrode setup. The observed mass activities (0.87±0.14 A mgPt−1) are close to the computational prediction (0.99 A mgPt−1). We report the highest to date mass activity among pure Pt catalysts for the ORR within similar size range. The specific and mass activities are twice as high as the Tanaka commercial Pt/C catalysis.}, keywords = {Foundry Organic, Solid-Liquid}, pubstate = {published}, tppubtype = {article} } Abstract High oxygen reduction (ORR) activity has been for many years considered as the key to many energy applications. Herein, by combining theory and experiment we prepare Pt nanoparticles with optimal size for the efficient ORR in proton-exchange-membrane fuel cells. Optimal nanoparticle sizes are predicted near 1, 2, and 3 nm by computational screening. To corroborate our computational results, we have addressed the challenge of approximately 1 nm sized Pt nanoparticle synthesis with a metal–organic framework (MOF) template approach. The electrocatalyst was characterized by HR-TEM, XPS, and its ORR activity was measured using a rotating disk electrode setup. The observed mass activities (0.87±0.14 A mgPt−1) are close to the computational prediction (0.99 A mgPt−1). We report the highest to date mass activity among pure Pt catalysts for the ORR within similar size range. The specific and mass activities are twice as high as the Tanaka commercial Pt/C catalysis. |
| 1. | K Trofymchuk, V Glembockyte, L Grabenhorst, F Steiner, C Vietz, C Close, M Pfeiffer, L Richter, M L Schütte, F Selbach, R Yaadav, J Zähringer, Q Wei, A Ozcan, B Lalkens, G P Acuna, P Tinnefeld Addressable Nanoantennas with Cleared Hotspots for Single-Molecule Detection on a Portable Smartphone Microscope Journal Article bioRxiv, pp. 2020.04.09.032037, 0000. Abstract | Links | Tags: Foundry Organic, Molecularly-Functionalized @article{, title = {Addressable Nanoantennas with Cleared Hotspots for Single-Molecule Detection on a Portable Smartphone Microscope}, author = {K Trofymchuk and V Glembockyte and L Grabenhorst and F Steiner and C Vietz and C Close and M Pfeiffer and L Richter and M L Sch\"{u}tte and F Selbach and R Yaadav and J Z\"{a}hringer and Q Wei and A Ozcan and B Lalkens and G P Acuna and P Tinnefeld}, url = {http://biorxiv.org/content/early/2020/04/09/2020.04.09.032037.abstract}, doi = {10.1101/2020.04.09.032037}, journal = {bioRxiv}, pages = {2020.04.09.032037}, abstract = {The advent of highly sensitive photodetectors1,2 and the development of photostabilization strategies3 made detecting the fluorescence of a single molecule a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g. in point-of-care diagnostic settings, where costly equipment would be prohibitive.4 Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in the NACHOS emit up to 461-fold brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia "on the road “.Competing Interest StatementPT and GPA are inventors on a patent of the described Bottom-up method for fluorescence enhancement in molecular assays, EP1260316.1, 2012, US20130252825 A1.}, keywords = {Foundry Organic, Molecularly-Functionalized}, pubstate = {published}, tppubtype = {article} } The advent of highly sensitive photodetectors1,2 and the development of photostabilization strategies3 made detecting the fluorescence of a single molecule a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g. in point-of-care diagnostic settings, where costly equipment would be prohibitive.4 Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in the NACHOS emit up to 461-fold brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia "on the road “.Competing Interest StatementPT and GPA are inventors on a patent of the described Bottom-up method for fluorescence enhancement in molecular assays, EP1260316.1, 2012, US20130252825 A1. |
Publications2020-03-26T14:18:16+01:00